MYOCYTE CELLULAR HYPERPLASIA AND MYOCYTE CELLULAR HYPERTROPHY CONTRIBUTE TO CHRONIC VENTRICULAR REMODELING IN CORONARY-ARTERY NARROWING-INDUCED CARDIOMYOPATHY IN RATS

被引:110
作者
KAJSTURA, J
ZHANG, X
REISS, K
SZOKE, E
LI, P
LAGRASTA, C
CHENG, W
DARZYNKIEWICZ, Z
OLIVETTI, G
ANVERSA, P
机构
[1] NEW YORK MED COLL, DEPT MED, VALHALLA, NY 10595 USA
[2] NEW YORK MED COLL, DEPT PHYSIOL, VALHALLA, NY 10595 USA
[3] UNIV PARMA, DEPT PATHOL, PARMA, ITALY
[4] ALBERT EINSTEIN COLL MED, DEPT MED, DIV CARDIOL, NEW YORK, NY USA
关键词
DNA SYNTHESIS; MYOCYTE MITOTIC DIVISION; MYOCYTE NUMBER; MYOCYTE SIZE; PLOIDY FORMATION; CARDIAC HYPERTROPHY;
D O I
10.1161/01.RES.74.3.383
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To determine whether cardiac failure produced by chronic coronary artery stenosis was associated with the activation of myocyte cellular hyperplasia in the myocardium, the changes in number and size of left ventricular myocytes were measured in rats 3 months after surgery. The hypertrophied left ventricle was found to possess 44%, 32%, 49%, and 48% fewer mononucleated, binucleated, trinucleated, and tetranucleated myocytes, respectively. In contrast, the hypertrophied right ventricle contained 1.49x10(6) more myocytes as a result of a 2.1-fold, 1.4-fold, and 1.4-fold increase in mononucleated, binucleated, and tetranucleated myocytes. Myocyte cell volume was seen to increase 49% and 21% in left and right ventricular myocytes, respectively. The process of myocyte cellular hyperplasia in the right ventricular myocardium was accompanied by capillary proliferation, and these events were responsible for the parallel addition of newly formed cells and capillaries within the wall and mural thickening. Moreover, the in-series insertion of new myocytes contributed to right ventricular dilation after coronary artery stenosis. In view of the fact that extensive myocardial damage and cell loss may have masked the phenomenon of myocyte cellular hyperplasia in the left ventricle, the presence of DNA synthesis in myocyte nuclei was evaluated at 3 days, 1 week, 2 weeks, 1 month, and 3 months after coronary artery stenosis. Bromodeoxyuridine (BrdU) labeling markedly increased in myocyte nuclei of both ventricles, reaching its peak at 1 and 2 weeks. BrdU labeling of nonmyocyte nuclei also increased but mostly at 2 weeks. To exclude that DNA synthesis was not coupled with ploidy formation, ploidy classes in myocytes were measured by flow cytometry and found not to be increased at 3 months after coronary artery narrowing in both ventricles. In addition, it was documented that mitosis occurred in myocytes and that this process was not associated with a change in the number of nuclei in myocytes. In conclusion, myocyte loss, myocyte cellular hypertrophy, and myocyte cellular hyperplasia all contribute to the development of the cardiomyopathic heart of ischemic origin. (Circ Res. 1994;74:383-400.)
引用
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页码:383 / 400
页数:18
相关论文
共 58 条
  • [1] ADLER C-P, 1971, Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin, V77, P1252
  • [2] MYOCARDIAL DNA CONTENT, PLOIDY LEVEL AND CELL NUMBER IN GERIATRIC HEARTS - POSTMORTEM EXAMINATIONS OF HUMAN MYOCARDIUM IN OLD-AGE
    ADLER, CP
    FRIEDBURG, H
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1986, 18 (01) : 39 - 53
  • [3] Adler CP, 1991, DEV REGENERATIVE POT, P227
  • [4] MYOCYTE MITOTIC DIVISION IN THE AGING MAMMALIAN RAT-HEART
    ANVERSA, P
    FITZPATRICK, D
    ARGANI, S
    CAPASSO, JM
    [J]. CIRCULATION RESEARCH, 1991, 69 (04) : 1159 - 1164
  • [5] ANVERSA P, 1978, LAB INVEST, V38, P597
  • [6] HYPERTENSIVE CARDIOMYOPATHY - MYOCYTE NUCLEI HYPERPLASIA IN THE MAMMALIAN RAT-HEART
    ANVERSA, P
    PALACKAL, T
    SONNENBLICK, EH
    OLIVETTI, G
    CAPASSO, JM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1990, 85 (04) : 994 - 997
  • [7] EFFECTS OF HYPERTENSION AND CORONARY CONSTRICTION ON CARDIAC-FUNCTION, MORPHOLOGY, AND CONTRACTILE PROTEINS IN RATS
    ANVERSA, P
    LI, P
    MALHOTRA, A
    ZHANG, X
    HERMAN, MV
    CAPASSO, JM
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02): : H713 - H724
  • [8] ISCHEMIC MYOCARDIAL INJURY AND VENTRICULAR REMODELING
    ANVERSA, P
    LI, P
    ZHANG, X
    OLIVETTI, G
    CAPASSO, JM
    [J]. CARDIOVASCULAR RESEARCH, 1993, 27 (02) : 145 - 157
  • [9] QUANTITATIVE STRUCTURAL-ANALYSIS OF THE MYOCARDIUM DURING PHYSIOLOGICAL GROWTH AND INDUCED CARDIAC-HYPERTROPHY - A REVIEW
    ANVERSA, P
    RICCI, R
    OLIVETTI, G
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1986, 7 (05) : 1140 - 1149
  • [10] CHRONIC CORONARY-ARTERY CONSTRICTION LEADS TO MODERATE MYOCYTE LOSS AND LEFT-VENTRICULAR DYSFUNCTION AND FAILURE IN RATS
    ANVERSA, P
    ZHANG, X
    LI, P
    CAPASSO, JM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (02) : 618 - 629