PURIFICATION AND PARTIAL CHARACTERIZATION OF ISOFORMS OF LUTEINIZING-HORMONE FROM THE CHICKEN PITUITARY-GLAND

被引：48

作者：

KRISHNAN, KA

PROUDMAN, JA

BAHR, JM

机构：

[1] USDA ARS, BELTSVILLE AGR RES CTR, GERMPLASM & GAMATE PHYSIOL LAB, BELTSVILLE, MD 20705 USA

[2] UNIV ILLINOIS, DEPT ANIM SCI, URBANA, IL 61801 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 108卷 / 02期

关键词：

LUTEINIZING HORMONE; CHICKEN PITUITARY GLAND;

D O I：

10.1016/0305-0491(94)90073-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isoforms of chicken luteinizing hormone (cLH) were isolated from chicken pituitaries by differential extraction, sequential chromatography on HPLC cation- and anion-exchange columns, and gel-filtration chromatography. Three purified isoforms of the cLH had high biological potency in the chicken granulosa cell LH bioassay (4.21-7.4 x NIH-LH-S1 U/mg). One cLH isoform without detectable biological activity showed substantial immunological activity in a homologous cLH radioimmunoassay. The cLH isoforms contained negligible follicle-stimulating hormone activity, but some contained thyroid-stimulating hormone activity. The apparent molecular weight of cLH was 37,000 Da, and the holoprotein consisted of subunits with a molecular weight of approximately 17,000 Da.

引用

页码：253 / 264

页数：12

共 29 条

[1] CIRCULATORY HALF-LIFE BUT NOT INTERACTION WITH THE LUTROPIN CHORIONIC-GONADOTROPIN RECEPTOR IS MODULATED BY SULFATION OF BOVINE LUTROPIN OLIGOSACCHARIDES [J].