PRESERVATION OF PROTEIN PHOSPHORYL GROUPS IN IMMUNOPRECIPITATION ASSAYS

被引：13

作者：

PAPAVASSILIOU, AG

机构：

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 170卷 / 01期

关键词：

IMMUNOPRECIPITATION; PHOSPHORYLATION; PHOSPHATASES; PHOSPHATASE INHIBITOR;

D O I：

10.1016/0022-1759(94)90246-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A phosphorylation-sensitive immunoprecipitation protocol is presented which ensures quantitative and qualitative recovery of phosphoantigens from different phosphatase environments. The protocol is based on the phosphatase-inactivating properties of a specific mixture of chemical compounds included in the ''classical'' immunoprecipitation buffers.

引用

页码：67 / 73

页数：7

共 20 条

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[2]

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[3]

BRAUTIGAN DL, 1988, METHOD ENZYMOL, V159, P339

[4]

Cotton FA., 1988, ADV INORG CHEM, V5