DISTRIBUTION OF TRANSMEMBRANE POLYPEPTIDES IN FREEZE-FRACTURE

被引：64

作者：

EDWARDS, HH

MUELLER, TJ

MORRISON, M

机构：

来源：

SCIENCE | 1979年 / 203卷 / 4387期

关键词：

D O I：

10.1126/science.424755

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human erythrocytes have been freeze-fractured, and the polypeptides associated with the separate halves of the membrane bilayer have been analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The transmembrane proteins were differentially separated by the fracture process. Although sialoglycoproteins associated with the outer half of the membrane, the anion transport protein (band 3) mainly remained with the inner half of the membrane. Well-defined fragments of the sialoglycoproteins were produced by the freeze-fracture procedure, indicating that selected covalent bonds of these transmembrane proteins were broken. Copyright © 1979 AAAS.

引用

页码：1343 / 1346

页数：4

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