EFFECT OF AGING ON THE SYNTHESIS OF HEXOSAMINE-CONTAINING SUBSTANCES FROM RAT COSTAL CARTILAGE - DECREASE IN SULFATION OF CHONDROITIN SULFATE WITH AGING

The amount of glycosaminoglycan (GAG) in dry costal cartilage tissue of rats decreased with aging, while the GAG content in mg DNA (unit cartilage cell) remained the same with aging. These results can be explained by the finding that the total number of cartilage cells decreased with aging.Electrophoretic analysis showed that chondroitin 4-sulfate was the major GAG in rat costal cartilage of various ages.Rat costal cartilage of different ages was incubated with radioactive precursors, and newly synthesized GAG was prepared and the radioactivity analyzed to determine the biosynthetic activity. As to changes in the radioactivity uptake with aging per mg dry cartilage tissue, aging influenced P incorporation into GAG more significantly than [3H]glucosamine incorporation into GAG. There was a significant decrease in the specific radioactivity of [35S] sulfate per mg DNA (unit cartilage cell), whereas the specific radioactivity of [3H]glucosamine per mg DNA did not change significantly with aging.Both the total sulfotransferase activity and the specific activity per mg DNA decreased significantly with aging.Analysis of disaccharide units formed after chondroitinase ABC digestion of labeled GAG isolated from young and old cartilage showed that the percentage of incorporation of [3H] glucosamine into ΔDi-0S increased significantly with aging. These results suggested that the appearance of nonsulfated positions in the structure of the chondroitin sulfate chain increased with aging.On the basis of gel chromatography on Bio-Gel A-l·5 m. no significant difference in the approximate molecular size of chondroitin sulfate was observed between the young and old GAG samples.The present study indicated that the sulfation of chondroitin sulfate chains from rat costal cartilage decreased with the process of aging. © 1979 By The Journal Of Biochemistry.