OVEREXPRESSION OF GENES OF AN EXTREME THERMOPHILE, THERMUS-THERMOPHILUS, IN ESCHERICHIA-COLI-CELLS

被引：18

作者：

ISHIDA, M ^{[1
]}

OSHIMA, T ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, FAC BIOSCI & BIOTECHNOL, DEPT LIFE SCI, YOKOHAMA, KANAGAWA 227, JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 09期

关键词：

D O I：

10.1128/JB.176.9.2767-2770.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli.

引用

页码：2767 / 2770

页数：4

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