A SHORT-TIME REFOLDING PROCEDURE THAT DRAMATICALLY INCREASES THE YIELDS OF RECOMBINANT MONOMERIC CARP GROWTH-HORMONE AND ITS CYS123ALA MUTANT - IMPLICATIONS FOR OTHER PROTEINS WITH AN UNPAIRED NUMBER OF CYSTEINE RESIDUES

被引：5

作者：

FINE, M

DANIEL, V

LEVANON, A

GERTLER, A

机构：

[1] HEBREW UNIV JERUSALEM,FAC AGR,DEPT BIOCHEM FOOD SCI & NUTR,IL-76100 REHOVOT,ISRAEL

[2] WEIZMANN INST SCI,DEPT BIOCHEM,IL-76100 REHOVOT,ISRAEL

[3] BIOTECHNOL GEN LTD,IL-76326 REHOVOT,ISRAEL

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 11期

关键词：

D O I：

10.1007/BF00153742

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant carp growth hormone and its Cys123Ala analogue were refolded in 4.5 M urea, pH 11.3 in the presence of 0.1 mM cysteine. Shortening of the refolding process from 48 h to 1 h resulted in a 30 to 40 fold increase in yield of the biologically active monomers, and lowered dimerization and oligomerization. A similar short-time refolding procedure was also found to be advantageous with other structurally different, non-related proteins, such as the extracellular domains of rabbit and bovine prolactin receptors.

引用

收藏

页码：769 / 774

页数：6

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