COMBINED ALKALI EXTRACTION - ETHIDIUM-BROMIDE TECHNIQUE FOR THE MEASUREMENT OF DNA IN SMALL PIECES OF TISSUE

被引：28

作者：

HALPRIN, KM ^{[1
]}

TAYLOR, JR ^{[1
]}

LEVINE, V ^{[1
]}

ADACHI, K ^{[1
]}

机构：

[1] UNIV MIAMI, SCH MED, DEPT DERMATOL, MIAMI, FL 33152 USA

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 1979年 / 73卷 / 05期

关键词：

D O I：

10.1111/1523-1747.ep12550415

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Alkaline solutions (0.1-0.5 N NaOH) at elevated temperatures can be used to extract DNA from small pieces of tissue. RNA is destroyed by the treatment. In tissues which were previously exposed to tritiated thymidine, aliquots from the extracting solution can be used directly for the determination of DNA by ethidium bromide fluorescence and radioactivity in DNA and the nucleotide pool. [Guinea pig and human skin were studied.].

引用

页码：359 / 363

页数：5

共 8 条

[1] KISSANE JM, 1958, J BIOL CHEM, V233, P184
[2] A FLUORESCENT COMPLEX BETWEEN ETHIDIUM BROMIDE AND NUCLEIC ACIDS - PHYSICAL-CHEMICAL CHARACTERIZATION
LEPECQ, JB
PAOLETTI, C
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1967, 27 (01) : 87 - +
[3] A NEW FLUOROMETRIC METHOD FOR RNA AND DNA DETERMINATION
LEPECQ, JB
PAOLETTI, C
[J]. ANALYTICAL BIOCHEMISTRY, 1966, 17 (01) : 100 - +
[4] ALKALI UNWINDING KINETICS OF MAMMALIAN DNA IN A SIMULATED VISCOELASTOMETRY EXPERIMENT
LINN, JD
WHEELER, KT
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 66 (02) : 712 - 716
[5] FORMATION OF TRIPLE-STRANDED BOVINE DNA INVITRO
PERLGUT, LE
BYERS, DL
JOPE, RS
KHAMVINWATHNA, V
[J]. NATURE, 1975, 254 (5495) : 86 - 87
[6] PRASAD AS, 1972, J LAB CLIN MED, V80, P598
[7] COMPLEX FORMATION BETWEEN ETHIDIUM BROMIDE AND NUCLEIC ACIDS
WARING, MJ
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1965, 13 (01) : 269 - &
[8] IS CYCLIC-AMP IN PSORIATIC EPIDERMIS LOW
YOSHIKAWA, K
ADACHI, K
HALPRIN, KM
LEVINE, V
[J]. BRITISH JOURNAL OF DERMATOLOGY, 1975, 93 (03) : 253 - 258

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