DIFFERENT FLEXIBILITY OF THE UPSTREAM REGULATORY REGIONS OF 2 DIFFERENTLY EXPRESSED PEA RBCS GENES STUDIED BY THEORETICAL EVALUATION OF DNA DISTORTION ENERGY AND CYCLIZATION KINETICS

被引：3

作者：

CACCHIONE, S

DESANTIS, P

SAVINO, M

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,P A MORO 5,I-00185 ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,DIPARTIMENTO CHIM,I-00185 ROME,ITALY

来源：

FEBS LETTERS | 1993年 / 336卷 / 02期

关键词：

SEQUENCE DEPENDENT DNA SUPERSTRUCTURE; DNA DISTORTION ENERGY CALCULATION; CYCLIZATION KINETICS; RBCS GENE;

D O I：

10.1016/0014-5793(93)80823-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Different superstructural features of the upstream regulatory regions of the two pea genes rbcS-3A and rbcS-E9 have been derived using a theoretical method, developed in our laboratory a few years ago, which evaluates the DNA distortion energy from a matrix of the deviations of the 16 possible dinucleotides from the standard B conformation. The theoretical analysis, which predicts different flexibilities of the regulatory regions of the two genes, is in satisfactorily good agreement with experimental evaluations from gel electrophoretic mobility and cyclization kinetics, suggesting a possible model to explain the largely different transcription efficiencies of the two genes.

引用

页码：293 / 298

页数：6

共 23 条

[1] THEORETICAL PREDICTION OF SEQUENCE-DEPENDENT DNA SUPERSTRUCTURES AND THEIR IMPLICATIONS IN RECOGNITION MECHANISMS WITH PROTEINS [J].