DIFFERENT FLEXIBILITY OF THE UPSTREAM REGULATORY REGIONS OF 2 DIFFERENTLY EXPRESSED PEA RBCS GENES STUDIED BY THEORETICAL EVALUATION OF DNA DISTORTION ENERGY AND CYCLIZATION KINETICS

被引：3

作者：

CACCHIONE, S

DESANTIS, P

SAVINO, M

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,P A MORO 5,I-00185 ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,DIPARTIMENTO CHIM,I-00185 ROME,ITALY

来源：

FEBS LETTERS | 1993年 / 336卷 / 02期

关键词：

SEQUENCE DEPENDENT DNA SUPERSTRUCTURE; DNA DISTORTION ENERGY CALCULATION; CYCLIZATION KINETICS; RBCS GENE;

D O I：

10.1016/0014-5793(93)80823-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Different superstructural features of the upstream regulatory regions of the two pea genes rbcS-3A and rbcS-E9 have been derived using a theoretical method, developed in our laboratory a few years ago, which evaluates the DNA distortion energy from a matrix of the deviations of the 16 possible dinucleotides from the standard B conformation. The theoretical analysis, which predicts different flexibilities of the regulatory regions of the two genes, is in satisfactorily good agreement with experimental evaluations from gel electrophoretic mobility and cyclization kinetics, suggesting a possible model to explain the largely different transcription efficiencies of the two genes.

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收藏

页码：293 / 298

页数：6

相关论文

共 23 条

[11] RELATIONSHIPS BETWEEN INTRINSIC AND INDUCED CURVATURE IN DNAS - THEORETICAL PREDICTION OF NUCLEOSOME POSITIONING [J].

DESANTIS, P ;

FUA, M ;

PALLESCHI, A ;

SAVINO, M .

BIOPHYSICAL CHEMISTRY, 1993, 46 (02) :193-204

[12]

DESANTIS P, 1988, BIOL ARTIFICIAL INTE, P143

[13] EXPRESSION DYNAMICS OF THE PEA RBCS MULTIGENE FAMILY AND ORGAN DISTRIBUTION OF THE TRANSCRIPTS [J].

FLUHR, R ;

MOSES, P ;

MORELLI, G ;

CORUZZI, G ;

CHUA, NH .

EMBO JOURNAL, 1986, 5 (09) :2063-2071

[14] ORGAN-SPECIFIC AND LIGHT-INDUCED EXPRESSION OF PLANT GENES [J].

FLUHR, R ;

KUHLEMEIER, C ;

NAGY, F ;

CHUA, NH .

SCIENCE, 1986, 232 (4754) :1106-1112

[15] SYNTHETIC DNA BENDING SEQUENCES INCREASE THE RATE OF INVITRO TRANSCRIPTION INITIATION AT THE ESCHERICHIA-COLI LAC-PROMOTER [J].

GARTENBERG, MR ;

CROTHERS, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (02) :217-230

[16] BINDING-SITE REQUIREMENTS FOR PEA NUCLEAR-PROTEIN FACTOR GT-1 CORRELATE WITH SEQUENCES REQUIRED FOR LIGHT-DEPENDENT TRANSCRIPTIONAL ACTIVATION OF THE RBCS-3A GENE [J].

GREEN, PJ ;

YONG, MH ;

CUOZZO, M ;

KANOMURAKAMI, Y ;

SILVERSTEIN, P ;

CHUA, NH .

EMBO JOURNAL, 1988, 7 (13) :4035-4044

[17] LOCALIZATION AND CONDITIONAL REDUNDANCY OF REGULATORY ELEMENTS IN RBCS-3A, A PEA GENE ENCODING THE SMALL SUBUNIT OF RIBULOSE-BISPHOSPHATE CARBOXYLASE [J].

KUHLEMEIER, C ;

CUOZZO, M ;

GREEN, PJ ;

GOYVAERTS, E ;

WARD, K ;

CHUA, NH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4662-4666

[18] UPSTREAM SEQUENCES DETERMINE THE DIFFERENCE IN TRANSCRIPT ABUNDANCE OF PEA RBCS GENES [J].

KUHLEMEIER, C ;

FLUHR, R ;

CHUA, NH .

MOLECULAR & GENERAL GENETICS, 1988, 212 (03) :405-411

[19] UPSTREAM CURVED SEQUENCES INFLUENCE THE INITIATION OF TRANSCRIPTION AT THE ESCHERICHIA-COLI GALACTOSE OPERON [J].

LAVIGNE, M ;

HERBERT, M ;

KOLB, A ;

BUC, H .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (02) :293-306

[20] INFLUENCE OF FLUCTUATIONS ON DNA CURVATURE - A COMPARISON OF FLEXIBLE AND STATIC WEDGE MODELS OF INTRINSICALLY BENT DNA [J].

OLSON, WK ;

MARKY, NL ;

JERNIGAN, RL ;

ZHURKIN, VB .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (02) :530-551