CLONING AND SEQUENCING OF THE GENE FOR THE LACTOSE CARRIER OF CITROBACTER-FREUNDII

被引：18

作者：

LEE, JI

OKAZAKI, N

TSUCHIYA, T

WILSON, TH

机构：

[1] HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115

[2] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT MICROBIOL,OKAYAMA 700,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2407

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for the lactose carrier of Citrobacter freundii was cloned into the plasmid pBR322. The gene was sequenced and the amino acid sequence was found to be 70% identical to the lactose carrier of E. coli. All of the charged residues in the membrane spanning region were conserved. The sugar specificity is somewhat different from that of E. coli. The C. freundii carrier has less activity for lactose and more activity for o-nitrophenylgalactose (ONPG) than the carrier of E. coli. (C) 1994 Academic Press. Inc.

引用

页码：1882 / 1888

页数：7

共 15 条

[1] NUCLEOTIDE-SEQUENCES AND OPERON STRUCTURE OF PLASMID-BORNE GENES MEDIATING UPTAKE AND UTILIZATION OF RAFFINOSE IN ESCHERICHIA-COLI [J].