CORRELATION OF (6-4)PHOTOPRODUCT FORMATION WITH TRANSFORMING MUTATIONS IN UV-IRRADIATED HA-RAS

被引：5

作者：

YAGI, T ^{[1
]}

MORIMOTO, T ^{[1
]}

TAKEBE, H ^{[1
]}

机构：

[1] KYOTO UNIV,GRAD SCH MED,DEPT RADIAT GENET,SAKYO KU,KYOTO 60601,JAPAN

来源：

CARCINOGENESIS | 1995年 / 16卷 / 04期

关键词：

D O I：

10.1093/carcin/16.4.689

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Sites and types of mutations in relation to the amount of cyclobutane pyrimidine dimers (CPDs) and (6-4)photoproducts in UV-irradiated normal Ha-ms sequences were investigated, Mouse BALB/C 3T3 cells were transfected with W-irradiated pYN-mHras plasmids containing mouse normal Ha-ras sequences, and transformed foci developed, Direct DNA sequencing of the Ha-ras retrieved from the foci revealed that most mutations (23/24, 96%) took place at dipyrimidine sequences, and the C-->T transition at the 3'-cytosine in 5'-TC or 5'-CC sequences was predominant (17/24, 71%) in codons 12, 13 and 60, In codon 61, where 5'-TC or 5'-CC is absent, two mutations were found at: the 5'-TT sequence. More (6-4)photoproducts were produced than CPDs in codons 12, 13 and 60, and more CPDs were produced than (6-4)photoproducts in codon 61. These results suggest that (6-4)photoproducts are the major lesion leading to the mutations in the mouse Ha-ras sequence and subsequent transformation of BALB/C 3T3 cells.

引用

页码：689 / 695

页数：7

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