REPLICATION OF ADENOVIRUS DNA IN-VITRO IS ATP-INDEPENDENT

被引：11

作者：

PRONK, R ^{[1
]}

VANDRIEL, W ^{[1
]}

VANDERVLIET, PC ^{[1
]}

机构：

[1] UNIV UTRECHT,PHYSIOL CHEM LAB,3521 GG UTRECHT,NETHERLANDS

来源：

FEBS LETTERS | 1994年 / 337卷 / 01期

关键词：

ADENOVIRUS; ATP; DNA POLYMERASE; DNA REPLICATION; DIVALENT CATION;

D O I：

10.1016/0014-5793(94)80624-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10-fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+ could efficiently be used for DNA chain elongation on synthetic templates we hypothesize that Mg2+ is essential for the transition of initiation to elongation.

引用

页码：33 / 38

页数：6

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