BINDING OF CIS-DICHLORODIAMMINEPLATINUM(II) AND TRANS-DICHLORODIAMMINEPLATINUM(II) TO DNA - EVIDENCE FOR UNWINDING AND SHORTENING OF THE DOUBLE HELIX

被引：275

作者：

COHEN, GL ^{[1
]}

BAUER, WR ^{[1
]}

BARTON, JK ^{[1
]}

LIPPARD, SJ ^{[1
]}

机构：

[1] COLUMBIA UNIV,DEPT CHEM,NEW YORK,NY 10027

来源：

SCIENCE | 1979年 / 203卷 / 4384期

关键词：

D O I：

10.1126/science.370979

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The antitumor drug cis-dichlorodiammineplatinum(II) (cis-DDP) and the inactive trans isomer bind and produce cooperative changes in closed and nicked circular duplex DNA's. Covalent binding of both platinum complexes to the closed circular DNA alters the degree of supercoiling, presumably by disrupting and unwinding the double helix. Electron micrographs show the platinated DNA's to be shortened by up to 50 percent of their original length. At similar ratios of bound platinum per nucleotide, the electrophoretic mobilities of the DNA's in gels containing the dye ethidium bromide are the same for both isomers. The only detectable difference in the binding of the two platinum isomers is an increase in the electrophoretic mobility in nondye gels of closed circular DNA having small amounts of bound cis-DDP that is not apparent for the trans complex. Copyright © 1979 AAAS.

引用

页码：1014 / 1016

页数：3

共 14 条

[1] HEAVY-METAL NUCLEOTIDE INTERACTIONS .11. STEREOCHEMICAL AND ELECTRONIC EFFECTS IN ELECTROPHILIC ATTACK OF CIS-DIAMMINEPLATINUM(II) AND TRANS-DIAMMINEPLATINUM(II) ON 5'-GUANOSINE MONOPHOSPHATE AND POLYGUANYLATE IN AQUEOUS-SOLUTION [J].