DETECTION INVOLVING POST-CHROMATOGRAPHIC ADDITION OF REAGENTS

被引：17

作者：

LITTLE, CJ

WHATLEY, JA

DALE, AD

机构：

[1] Physical Methods Department, Roche Products Limited, Welwyn Garden City,, Herts. Herts.

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 171卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(01)95286-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Unlike the approach to reagent addition as used in AutoAnalyzer methodology, we have developed a packed bed mixer which can be used without recourse t a gas-segmented stream. The very low contribution of the mixer to the overall dispersion of the chromatographic peak is compatible with high-performance liquid chromatography. The mixer is particularly suitable for use with fast post-column reactions such as the fluorimetric determination of amines with fluorescamine or o-phthalaldehyde as reagents. The mixer is ideally suited for use in conjunction with electrochemical detection where addition of reagents to control pH and electrolyte composition is very important. © 1979.

引用

页码：63 / 72

页数：10

共 19 条

[1] CHROMATOGRAPHIC ANALYSIS OF AMINO-ACIDS AND PRIMARY AMINES WITH O-PHTHALALDEHYDE DETECTION [J].