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SEQUENCE-ANALYSIS OF THE SPLICE JUNCTION IN THE TRANSCRIPT OF HERPES-SIMPLEX VIRUS TYPE-1 GENE UL15

被引：24
作者：
DOLAN, A
ARBUCKLE, M
MCGEOCH, DJ
机构：
[1] Institute of Virology, University of Glasgow
来源：
VIRUS RESEARCH | 1991年 / 20卷 / 01期
关键词：
HERPES SIMPLEX VIRUS; SPLICED GENE; SPLICE SITE; GENE UL15;
D O I：
10.1016/0168-1702(91)90064-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Gene UL15 of herpes simplex virus type 1 has been proposed to consist of two coding exons separated by an intron of 3587 base pairs. We have generated a DNA fragment copied from the transcript across the proposed splice junction by successive use of reverse transcription and the polymerase chain reaction, and have sequenced this fragment to determine the precise structure of the splice junction.
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页码：97 / 104
页数：8
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