SEPARATION OF HYDROGEN-BONDING DONORS IN CAPILLARY ELECTROPHORESIS USING POLYETHERS AS MATRIX

被引：44

作者：

ESAKA, Y

YAMAGUCHI, Y

KANO, K

GOTO, M

HARAGUCHI, H

TAKAHASHI, J

机构：

[1] GIFU PHARMACEUT UNIV,GIFU 502,JAPAN

[2] NAGOYA UNIV,DEPT APPL CHEM,CHIKUSA KU,NAGOYA 464,JAPAN

[3] SEIKO INSTRUMENTS INC,DIV SCI INSTRUMENTS,MIHAMA KU,CHIBA 261,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 15期

关键词：

D O I：

10.1021/ac00087a006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Addition of poly(ethylene glycol) (PEG) as a free matrix was found to be a novel separation parameter in capillary zone electrophoresis. In the separation of substituted and unsubstituted benzoic acids used as model analytes, attractive interaction with PEG was observed for the analytes with hydroxyl, amide, or amine groups. This interaction is attributable to hydrogen bond formation. The concentration and the polymer length of PEG can control the net strength of the interaction and then the observed separation. This capillary electrophoresis using the hydrogen-bonding mode was successfully applied to the separation of nucleotides.

引用

页码：2441 / 2445

页数：5

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