MUTATIONS IN GLTC THAT INCREASE BACILLUS-SUBTILIS GLTA EXPRESSION

被引：18

作者：

BELITSKY, BR ^{[1
]}

SONENSHEIN, AI ^{[1
]}

机构：

[1] TUFTS UNIV,SCH MED,DEPT MOLEC BIOL & MICROBIOL,BOSTON,MA 02111

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 19期

关键词：

D O I：

10.1128/jb.177.19.5696-5700.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA became much less dependent on a nitrogen source-associated signal.

引用

页码：5696 / 5700

页数：5

共 24 条

[1] INTERACTIONS OF WILD-TYPE AND MUTANT AMPR OF CITROBACTER-FREUNDII WITH TARGET DNA [J].

BARTOWSKY, E ;

NORMARK, S .

MOLECULAR MICROBIOLOGY, 1993, 10 (03) :555-565

[2] SITES REQUIRED FOR GLTC-DEPENDENT REGULATION OF BACILLUS-SUBTILIS GLUTAMATE SYNTHASE EXPRESSION [J].

BELITSKY, BR ;

JANSSEN, PJ ;

SONENSHEIN, AL .

JOURNAL OF BACTERIOLOGY, 1995, 177 (19) :5686-5695

[3]

BELITSKY BR, UNPUB

[4] POSITIVE REGULATION OF GLUTAMATE BIOSYNTHESIS IN BACILLUS-SUBTILIS [J].

BOHANNON, DE ;

SONENSHEIN, AL .

JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4718-4727

[5] REGULATION OF BACILLUS-SUBTILIS GLUTAMATE SYNTHASE GENES BY THE NITROGEN-SOURCE [J].

BOHANNON, DE ;

ROSENKRANTZ, MS ;

SONENSHEIN, AL .

JOURNAL OF BACTERIOLOGY, 1985, 163 (03) :957-964

[6] ALTERED-FUNCTION MUTATIONS IN THE AGROBACTERIUM-TUMEFACIENS OCCR PROTEIN AND IN AN OCCR-REGULATED PROMOTER [J].

CHO, KY ;

WINANS, SC .

JOURNAL OF BACTERIOLOGY, 1993, 175 (23) :7715-7719

[7] RESIDUE THREONINE-149 OF THE SALMONELLA-TYPHIMURIUM CYSB TRANSCRIPTION ACTIVATOR - MUTATIONS CAUSING CONSTITUTIVE EXPRESSION OF POSITIVELY REGULATED GENES OF THE CYSTEINE REGULON [J].

COLYER, TE ;

KREDICH, NM .

MOLECULAR MICROBIOLOGY, 1994, 13 (05) :797-805

[8] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION [J].

DOWER, WJ ;

MILLER, JF ;

RAGSDALE, CW .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6127-6145

[9] FATE OF TRANSFORMING DNA FOLLOWING UPTAKE BY COMPETENT BACILLUS-SUBTILIS [J].

DUBNAU, D ;

DAVIDOFF.R .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 56 (02) :209-&

[10] A TARGET FOR CARBON SOURCE-DEPENDENT NEGATIVE REGULATION OF THE CITB PROMOTER OF BACILLUS-SUBTILIS [J].

FOUET, A ;

SONENSHEIN, AL .

JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :835-844

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