PREPARATION OF HIGH RESOLUTION MICROGRANULAR CARBOXYMETHYL CELLULOSE COLUMNS AND THEIR APPLICATION TO SEPARATION OF GUANETHIDINE AND ITS METABOLITES IN URINE

Colunms (1.2 × 6 cm) with up to 100 theoretical plates/cm were prepared using CM cellulose which had been graded by back-washing. The columns were fitted with specially made polythene column ends to allow samples to enter and leave the column bed with minimum distortion and were rapidly packed at high flow rates of buffer. The columns could be operated at low pressures and used repeatedly over several weeks. Four columns connected in series were used to separate radioactive products excreted in the urine of rats which had been treated with guanethidine. Four radio-active products which accounted for 99% of the applied radioactivity were identified as 1-(6.carboxyhexyl)-2-iminoimidazolidine, 2.(6-carboxyhexylamino)-ethylguanidine, guanethidine N-oxide and guanethidine. © 1969.