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PROTEIN-KINASE C-GAMMA IS PRESENT IN ADRIAMYCIN RESISTANT HL-60 LEUKEMIA-CELLS

被引：60
作者：
AQUINO, A
WARREN, BS
OMICHINSKI, J
HARTMAN, KD
GLAZER, RI
机构：
[1] NCI,DIV CANC ETIOL,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892
[2] UNIV TEXAS,MD CANC CTR,DEPT CARCINOGENESIS,SMITHVILLE,TX 78957
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90869-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The isoform pattern of protein kinase C (PKC) was examined in wild-type and Adriamycin-resistant ( HL-60 AR) HL-60 leukemia cells. Analyses were carried out by immunoblotting with mouse monoclonal antibodies against PKC-α and PKC-β and a rabbit polyclonal antibody against the variable (V3) region of PKC-γ. HL-60 AR cells contained an equivalent level of PKC-ga and a lower amount of PKC-β than HL-60 cells. In contrast, only HL-60 AR cells contained PKC-γ. These results indicate that the regulation of this family of isoenzymes is altered in drug-resistant cells. © 1990.
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页码：723 / 728
页数：6
相关论文
共 23 条
[1]  
AQUINO A, 1988, CANCER RES, V48, P3324
[2]  
BHALLA K, 1985, CANCER RES, V45, P3657
[3]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[4]   MULTIPLE, DISTINCT FORMS OF BOVINE AND HUMAN PROTEIN-KINASE-C SUGGEST DIVERSITY IN CELLULAR SIGNALING PATHWAYS [J].
COUSSENS, L ;
PARKER, PJ ;
RHEE, L ;
YANGFENG, TL ;
CHEN, E ;
WATERFIELD, MD ;
FRANCKE, U ;
ULLRICH, A .
SCIENCE, 1986, 233 (4766) :859-866
[5]   PHORBOL ESTERS INDUCE MULTIDRUG RESISTANCE IN HUMAN-BREAST CANCER-CELLS [J].
FINE, RL ;
PATEL, J ;
CHABNER, BA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :582-586
[6]  
FONTANA JA, 1986, CANCER RES, V46, P2468
[7]  
HUANG FL, 1987, J BIOL CHEM, V262, P15714
[8]   ISOZYMIC FORMS OF RAT-BRAIN CA-2+-ACTIVATED AND PHOSPHOLIPID-DEPENDENT PROTEIN-KINASE [J].
HUANG, KP ;
NAKABAYASHI, H ;
HUANG, FL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8535-8539
[9]   CLONING AND EXPRESSION OF MULTIPLE PROTEIN-KINASE-C CDNAS [J].
KNOPF, JL ;
LEE, MH ;
SULTZMAN, LA ;
KRIZ, RW ;
LOOMIS, CR ;
HEWICK, RM ;
BELL, RM .
CELL, 1986, 46 (04) :491-502
[10]   THE HETEROGENEITY OF PROTEIN KINASE-C IN VARIOUS RAT-TISSUES [J].
KOSAKA, Y ;
OGITA, K ;
ASE, K ;
NOMURA, H ;
KIKKAWA, U ;
NISHIZUKA, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (03) :973-981
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