IMMUNOCHEMICAL COMPARISON OF HISTIDINE-RICH PROTEIN IN KERATOHYALIN GRANULES AND CORNIFIED CELLS

1. (1)|Combination of techniques for extraction and purification of histidine rich protein established by several investigators were employed for comparison of histidine-rich protein in granular cells and cornified cells of newborn rats. 2. (2)|Histidine-rich protein extracted from the same cell fraction by two different techniques either in 1 M potassium phosphate buffer (Ugel) or in 4 M urea (Dale) showed identical elution profiles on CM 52 cellulose ion exchange chromatography and the same SDS polyacrylamide gel electrophoretic patterns. 3. (3)|Histidine-rich protein from granular cells contained polypeptides of larger molecular sizes than those in histidine-rich protein from cornified cells, although amino acid composition of the two histidine-rich protein was non-distinguishable (histidine residue was more than 7%). 4. (4)|Antibodies raised in rabbits by injection of histidine rich protein from granular cells and that from cornified cells immunologically cross-reacted. Furthermore, the antisera were found to be reactive over both keratohyalin granules and cornified cells, but not epidermal cells of the lower strata. © 1979.