ISOLATION AND EXPRESSION OF CDNA FOR DIFFERENT FORMS OF HEPATOCYTE GROWTH-FACTOR FROM HUMAN-LEUKOCYTE

被引：203

作者：

SEKI, T

IHARA, I

SUGIMURA, A

SHIMONISHI, M

NISHIZAWA, T

ASAMI, O

HAGIYA, M

NAKAMURA, T

SHIMIZU, S

机构：

[1] TOYOBO CO LTD, PHARMACEUT RES CTR, OTSU 52002, JAPAN

[2] KYUSHU UNIV, FAC SCI, DEPT BIOL, FUKUOKA 812, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80212-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe. Nucleotide and deduced amino acid sequences were analyzed for two of four clones obtained. One of them contained an open reading frame coding for a polypeptide chain of 728 amino acid residues like that of cDNA clone derived from human liver. In another clone a spontaneous deletion of 15 base pairs was found within the coding sequence. When expressed transiently using COS-1 cells both clones produced protein with similar biological activity against rat hepatocyte in vitro. © 1990 Academic Press, Inc.

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页码：321 / 327

页数：7

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