HATCHING RATE - AN OPTIMAL DISCRIMINATOR FOR THE ASSESSMENT OF SINGLE-BLASTOMERE BIOPSY

被引：16

作者：

CUI, KH

VERMA, PJ

MATTHEWS, CD

机构：

[1] Department of Obstetrics and Gynaecology, The University of Adelaide, The Queen Elizabeth Hospital, Woodville, 5011, S.A.

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 02期

关键词：

BLASTOCYST HATCHING; EMBRYO BIOPSY; EVALUATION OF TECHNIQUE; IN-VITRO FERTILIZATION; PREIMPLANTATION DIAGNOSIS;

D O I：

10.1007/BF01207740

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: In order to determine an optimal marker to discriminate embryo injury following single-blastomere embryo biopsy, mouse embryos were examined for rates of blastocyst formation, hatching, implantation, and fetal development following single-blastomere biopsy. Results: Early studies of single-blastomere biopsy (1-8 series) resulted in similar rates of blastocyst formation (P > 0.05) but a lower rate of hatching of biopsied (n = 140) versus control (nonbiopsied) (n = 145) embryos (78.6 vs 95.2%; p < 0.01). Subsequent experience (9-13 series) eliminated this difference between biopsied (n = 145) and control embryos (n = 133) (95.9 vs 94.0%; P > 0.05). Embryo transfer of hatching blastocysts of biopsied (n = 100) and nonbiopsied control (n = 100) groups resulted in equivalent rates of implantation (96.0 vs 92.0%; p > 0.05) and an equivalent rate of fetal development (70.0 vs 68.0%; p > 0.05). Conclusions: The hatching rate appeared to be a simple, sensitive, and reliable method to evaluate the single-blastomere biopsy technique.

引用

页码：157 / 162

页数：6

共 18 条

[1]

Verlinsky Y., Ginsberg N., Lifchez A., Valle J., Moise J., Strom C., Analysis of the first polar body: preconception genetic diagnosis, Hum Reprod, 5, pp. 826-829, (1990)

[2]

Monk M., Holding C., Amplification of a β-haemoglobin sequence in individual human oocytes and polar bodies, Lancet, 335, pp. 985-988, (1990)

[3]

Wilton L.J., Trounson A.O., Biopsy of preimplantation mouse embryos: Development of micromanipulated embryos and proliferation of single blastomeres in vitro, Biol Reprod, 40, pp. 145-152, (1989)

[4]

Handyside A.H., Pattinson J.K., Penketh R.J.A., Delhanty J.D.A., Winston R.M.L., Tuddenham E.G.D., Biopsy of human preimplantation embryos and sexing by DNA amplification, Lancet, 1, pp. 347-349, (1989)

[5]

Summers P.M., Campbell J.M., Miller M.W., Normal in-vivo development of marmoset monkey embryos after trophectoderm biopsy, Hum Reprod, 3, pp. 389-393, (1988)

[6]

Monk M., Muggleton-Harris A.L., Rawlings E., Whittingham D.G., Pre-implantation diagnosis of HPRT-deficient male and carrier female mouse embryos by trophectoderm biopsy, Hum Reprod, 3, pp. 377-381, (1988)

[7]

Peura T., Hyttinen J.M., Turunen M., Janne J., A reliable sex determination assay for bovine pre-implantation embryos using the polymerase chain reaction, Theriogenology, 35, pp. 547-555, (1991)

[8]

Roberts C., Lutjen J., Krzyminska U., O'Neill C., Cytogenetic analysis of biopsied preimplantation mouse embryos: Implications for prenatal diagnosis, Hum Reprod, 5, pp. 197-202, (1990)

[9]

Bowman P., McLaren A., Cleavage rate of mouse embryos in vivo and in vitro, J Embryol Exp Morphol, 24, pp. 203-207, (1970)

[10]

Streffer C., Van Beuningen D., Molls M., Zamboglou N., Schulz J., Kinetics of cell proliferation in the pre-implanted mouse embryo in vivo and in vitro, Cell Tissue Kinet, 13, pp. 135-143, (1980)

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