FACTORS THAT BIND TO RNA-POLYMERASE-I PROMOTER SEQUENCES OF TRYPANOSOMA-BRUCEI

被引:16
作者
JANZ, L [1 ]
HUG, M [1 ]
CLAYTON, C [1 ]
机构
[1] UNIV HEIDELBERG,ZENTRUM MOLEK BIOL,D-69120 HEIDELBERG,GERMANY
关键词
TRYPANOSOMA; GEL MOBILITY SHIFT; PROMOTER; TRANSCRIPTION;
D O I
10.1016/0166-6851(94)90119-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The procyclic acidic repetitive protein (procyclin) and variant surface glycoprotein genes of Trypanosoma brucei are transcribed by a polymerase sharing many features with RNA polymerase I. Mutational analyses on the PARP and ribosomal RNA promoters have shown that sequences important for promoter activity are concentrated 20-60 bp upstream of the transcription initiation site. The results of gel mobility shift assays using synthetic oligonucleotides spanning these regions indicated the presence in trypanosomal extracts of factors capable of binding each promoter in a highly specific fashion. There was no evidence that the PARP, VSG and rRNA promoter fragments bound the same factor.
引用
收藏
页码:99 / 108
页数:10
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