A DISTINCTIVE ELECTROPHYSIOLOGICAL SIGNATURE FROM THE PEYERS-PATCHES OF RABBIT INTESTINE

被引:15
作者
BRAYDEN, DJ [1 ]
BAIRD, AW [1 ]
机构
[1] UNIV DUBLIN TRINITY COLL, DEPT PHARMACOL, DUBLIN, IRELAND
关键词
RABBIT PEYERS PATCHES; USSING CHAMBER; CARBACHOL; M CELLS;
D O I
10.1111/j.1476-5381.1994.tb17031.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 Rabbit small intestinal segments containing Peyer's patches (PP) were examined in Ussing chambers using short-circuit current (I-sc) recording. By comparison with control small intestinal mucosal segments, rabbit PP-containing epithelia exhibited decreased basal I-sc, increased transepithelial resistance (TER) and unchanged potential difference (PD). 2 Carbachol caused a decrease in I-sc in rabbit PP epithelia. Forskolin, dibutyryl cyclic GMP, histamine and the calcium ionophore, A23187, were without effect. In contrast, control epithelial segments of rabbit intestine responded to carbachol and forskolin with an increased I-sc, indicative of electrogenic chloride secretion. The EC(50) for carbachol was approximately 2 mu M in both types of epithelia. Methacholine also caused an outward current in rabbit PP epithelia which had similar properties to that of carbachol. The effect of the cholinomimetics on rabbit PP was basolateral-sided, reversible, and sensitive to low concentrations of the general muscarinic cholinoceptor blockers, atropine, scopolamine and also to the M(1) cholinoceptor blocker, pirenzepine. 3 The I-sc response to cholinomimetics in rabbit PP was insensitive to bumetanide, amiloride, TEA, barium, acetazolamide, piroxicam and omeprazole, but was attenuated in the presence of ouabain. Using bilaterally-substituted solutions, the carbachol effect on rabbit PP I-sc was abolished in chloride/bicarbonate-free, but not in chloride-free solutions, suggestive of stimulation of electrogenic bicarbonate absorption by the agent. Substitution for sodium abolished both the basal current and the I-sc response to carbachol. Part of the effect of carbachol on PP I-sc appeared to be mediated by submucosal neurones because addition of tetrodotoxin reduced the effect by 60%. 4 As microfold (M) epithelial cells predominate in the PP of the rabbit, the unusual phenotype of cholinomimetic-induced outward current may be used as an electrophysiological marker for these potential sites of oral vaccine delivery, and in particular it may also be of use as a marker for rabbit M cells.
引用
收藏
页码:593 / 599
页数:7
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