CHARACTERIZATION OF A NEW CELL-BOUND ALPHA-AMYLASE IN BACILLUS-SUBTILIS 168-MARBURG THAT IS ONLY IMMUNOLOGICALLY RELATED TO THE EXOCELLULAR ALPHA-AMYLASE

被引：8

作者：

HADDAOUI, E

PETITGLATRON, MF

CHAMBERT, R

机构：

[1] Laboratoire Genetique et Membranes, Institut Jacques Monod, 75251 Paris Cedex 05, 2, Place Jussieu

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 17期

关键词：

D O I：

10.1128/jb.177.17.5148-5150.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, revealed one protein species of 82,000 Da. This protein was found even in cells in which the amyE gene, encoding exocellular alpha-amylase, was disrupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.

引用

页码：5148 / 5150

页数：3

共 9 条

[1]

CHAMBERT R, 1984, J GEN MICROBIOL, V130, P3143

[2] A NEOMYCIN RESISTANCE GENE CASSETTE SELECTABLE IN A SINGLE COPY STATE IN THE BACILLUS-SUBTILIS CHROMOSOME [J].