A TECHNIQUE FOR STUDY OF ACETYLCHOLINE TURNOVER IN MOUSE BRAIN IN VIVO

被引：165

作者：

SCHUBERTH, J

SPARF, B

SUNDWALL, A

机构：

[1] Research Institute of National Defence, Stockholm

[2] Psychiatric Research Center, Ulleråker Hospital, Uppsala

来源：

JOURNAL OF NEUROCHEMISTRY | 1969年 / 16卷 / 05期

关键词：

D O I：

10.1111/j.1471-4159.1969.tb06447.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— —A method to measure the rate of acetylcholine turnover in mouse brain in vivo has been developed. It is based on the formation of labelled acetylcholine from intravenously injected labelled choline. The isotopic dilution of choline in the brain has been measured by assaying endogenous choline in the brain by an enzymatic method using tritium‐labelled acetyl‐CoA and purified choline acetyltransferase. The rate of acetylcholine turnover in the brain could be calculated at 50 n‐moles acetylcholine/g/min in conscious mice. In anaesthetized mice and in mice treated with oxotremorine, a decrease of acetylcholine turnover to about 10 n‐moles/g/min was found. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：695 / +

页数：1

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