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PROTEIN COATED ADSORBENTS FOR USE IN POTENTIAL BARRIER CHROMATOGRAPHY - FOULING CHROMATOGRAPHY
被引：5
作者：
LESINS, V
RUCKENSTEIN, E
机构：
[1] State Univ of New York at Buffalo,, Buffalo, NY, USA, State Univ of New York at Buffalo, Buffalo, NY, USA
关键词：
ADSORBENTS;
-;
Applications;
ADSORPTION;
BIOTECHNOLOGY;
PROTEINS;
Separation;
D O I：
10.1002/btpr.5420040104
中图分类号：
Q81 [生物工程学（生物技术）];
Q93 [微生物学];
学科分类号：
071005 ;
0836 ;
090102 ;
100705 ;
摘要：
Fouling is employed as a method of obtaining adsorbents suitable for use in Potential Barrier Chromatography. A relatively inexpensive hydrophobic adsorbent, which is initially unsuitable for protein separations, is intentionally fouled with protein. The packing slowly evolves from an adsorbent which binds proteins strongly, due to strong hydrophobic interactions, to a protein coated adsorbent in which weaker hydrophobic interactions are opposed by enhanced double layer repulsion. As a result, an adsorbent which is suitable for protein separations evolves. Details regarding the fouling process, the chromatographic separation of two model proteins and the physical forces involved are outlined.
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页码：12 / 24
页数：13
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