EXPERIMENTAL-STUDY OF SELECTIVITY AND COLUMN EFFICIENCY IN CLATHRATE CHROMATOGRAPHY USING WERNER COMPLEXES AS CLATHRATE HOST COMPONENTS .2. OPTIMIZATION OF OPERATING PARAMETERS

The column efficiency has been studied in liquid chromatographic systems with β-Ni (NCS)2 (4-MePy)4 clathrates as teh stationary phase. It was found that peak broadening is determined mainly by the diffusion proceses within the clathrate crystals. The relative values of the diffusion coeficients of o-, m- and p-dinitrobenzene isomers as a function of clathrate structure parameters have been estimated using Giddings theory. It was found experimentallty that the optimum efficiency can be obtained with small particles of 5-10μm diameter and low flow-rates, less than 10 ml/h. This limitation in flow-rate is acceptable because of the high selectivity of the β-clathrate; thus 3-4 cm long columns are sufficiently efficient. © 1979.