GAS-CHROMATOGRAPHIC ANALYSIS OF MALONALDEHYDE AND 4-HYDROXY-2-(E)-NONENAL PRODUCED FROM ARACHIDONIC-ACID AND LINOLEIC-ACID IN A LIPID-PEROXIDATION MODEL SYSTEM

被引：58

作者：

TAMURA, H ^{[1
]}

SHIBAMOTO, T ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT ENVIRONM TOXICOL,DAVIS,CA 95616

来源：

LIPIDS | 1991年 / 26卷 / 02期

关键词：

D O I：

10.1007/BF02544014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Malonaldehyde (MA) and 4-hydroxynonenal (4-HN) formed upon oxidation with Fe2+/H2O2 from arachidonic acid and linoleic acid, and their ethyl esters were analyzed by gas chromatography (GC). The MA and 4-HN produced were reacted with N-methylhydrazine (NMH) to give 1-methylpyrazole and 5(1'-hydroxyhexyl)-1-methyl-2-pyrazoline, respectively. The derivatives were analyzed by GC on a fused silica capillary column using a nitrogen-phosphorus detector. With arachidonic acid, more MA and 4-HN were formed from the ester (88 nmol/mg and 23 nmol/mg, respectively) than from the free acid (25 nmol/mg and 9 nmol/mg, respectively). In contrast, with linoleic acid, more MA and 4-HN were produced from the free acid (53 nmol/mg and 13 nmol/mg, respectively) than from the ester (39 nm/mg and 8 nmol/mg, respectively).

引用

页码：170 / 173

页数：4

共 18 条

[1] IDENTIFICATION OF 4-HYDROXYNONEAL AS A CYTO-TOXIC PRODUCT ORIGINATING FROM THE PEROXIDATION OF LIVER MICROSOMAL LIPIDS [J].