EFFECT OF TURNING UPON THE SUB-EMBRYONIC FLUID AND ALBUMIN OF THE EGG OF THE JAPANESE-QUAIL

1. The effects of egg turning upon the formation of sub-embryonic fluid (SEF), and albumen weight and composition are described up to day 8 of incubation. 2. The density of albumen increased, and the density of yolk sac contents decreased, during the first 5 d of incubation. Failure to turn eggs diminished these changes. 3. The rate of formation of SEF and its maximum weight were lower if eggs were not turned, as was the rate of decrease in albumen weight. 4. The concentration of sodium and chloride, as well as osmolality, were higher in SEF than in thin albumen, and were affected by a failure to turn eggs. 5. Static incubation altered the concentrations of yolk nutrients in SEF. 6. It is-concluded that the formation of SEF is primarily dependent upon the transfer of sodium and chloride from albumen to SEF so creating an osmotic force for water movement in the same direction. Turning the eggs promotes this process by ensuring an adequate supply of ions in thin albumen adjacent to the blastoderm.