RECENT ADVANCES IN ANALYTICAL METHODS FOR MYCOTOXINS

被引：30

作者：

GILBERT, J

机构：

[1] Food Science Laboratory, Ministry of Agriculture, Fisheries and Food, Colney, Norwich, NR4 7UQ, Norwich Research Park

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1993年 / 10卷 / 01期

关键词：

MYCOTOXINS; AFLATOXINS; TRICHOTHECENES; HPLC; LC MS; MS MS; SFC MS; ANALYSIS;

D O I：

10.1080/02652039309374128

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Recent advances in analytical methods are reviewed using the examples of aflatoxins and trichothecene mycotoxins. The most dramatic advances are seen as being those based on immunological principles utilized for aflatoxins to produce simple screening methods and for rapid specific clean-up. The possibilities of automation using immunoaffinity columns is described. In contrast for the trichothecenes immunological methods have not had the same general impact. Post-column derivatization using bromine or iodine to enhance fluorescence for HPLC detection of aflatoxins has become widely employed and there are similar possibilities for improved HPLC detection for trichothecenes using electrochemical or trichothecene-specific post-column reactions. There have been improvements in the use of more rapid and specific clean-up methods for trichothecenes, whilst HPLC and GC remain equally favoured for the end-determination. More sophisticated instrumental techniques such as mass spectrometry (LC/MS, MS/MS) and supercritical fluid chromatography (SFC/MS) have been demonstrated to have potential for application to mycotoxin analysis, but have not as yet made much general impact.

引用

页码：37 / 48

页数：12

共 41 条

[11]

Koeltzow D.E., Tanner S.N., Comparative evaluation of commercially available aflatoxin test methods, Journal of the Association of Official Analytical Chemists, 73, pp. 584-589, (1990)

[12]

Kok W.T.H., Van Neer C.H.T.H., Traag W.A., Tuinstra L., Determination of aflatoxins in cattle feed by liquid chromatography and post-column derivatization with electrochemically generated bromide, Journal of Chromatography, 367, pp. 231-236, (1986)

[13]

Krishnamurthy T., Sarver E.W., Mass spectral investigations on trichothecene mycotoxins, Biomedical and Environmental Mass Spectrometry, 15, pp. 185-191, (1988)

[14]

Lauren D.R., Greenhalgh R., Simultaneous analysis of nivalenol and deoxynivalenol in cereals by liquid chromatography, Journal of the Association of Official a Nalytical Chemists, 70, pp. 479-483, (1987)

[15]

Mortimer D.N., Gilbert J., Shepherd M.J., Rapid and highly sensitive analysis of aflatoxin M1 in liquid and powdered milks using affinity column cleanup, Journal Of, 407, pp. 393-398, (1987)

[16]

Nagayama S., Kawamura O., Ohtani K., Ryu J.-C., Latus D., Sudheim L., Ueno Y., Application of an enzyme-linked immunosorbent assay for screening T-2 toxin-producing Fusarium spp, Applied and Environmental Microbiology, 54, pp. 1302-1303, (1988)

[17]

Patey A.L., Sharman M., Wood R., Gilbert J., Determination of aflatoxin concentrations in peanut butter by enzyme-linked immunosorbent assay (ELISA): Study of three commercial ELISA kits, Journal of the Association of Official Analytical Chemists, 72, pp. 965-969, (1989)

[18]

Plattner R.D., Bennett G.A., Rapid detection of Fusarium mycotoxins in grains by quadruple MS/MS, Journal of the Association of Official Analytical Chemists, 66, pp. 1470-1477, (1983)

[19]

Plattner R.D., Bennett G.A., Stubblefield R.D., Identification of aflatoxins by quadrupole MS/MS, Journal of the Association of Official Analytical Chemists, 67, pp. 734-738, (1984)

[20]

Plattner R.D., Yates S.G., Porter J.K., Quadrupole MS/MS of ergot cyclol alkaloids, Journal of Agricultural and Food Chemistry, 31, pp. 785-789, (1983)

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