CLONING OF HUMAN AND RAT CDNAS ENCODING THE MITOCHONDRIAL SINGLE-STRANDED DNA-BINDING PROTEIN (SSB)

被引：96

作者：

TIRANTI, V

ROCCHI, M

DIDONATO, S

ZEVIANI, M

机构：

[1] IST NAZL NEUROL C BESTA,DIV BIOCHIM & GENET,VIA CELORIA 11,I-20133 MILAN,ITALY

[2] UNIV BARI,IST GENET MED,I-70124 BARI,ITALY

来源：

GENE | 1993年 / 126卷 / 02期

关键词：

RECOMBINANT DNA; NUCLEAR GENE; DNA REPLICATION; PRESEQUENCE; HUMAN CHROMOSOME;

D O I：

10.1016/0378-1119(93)90370-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have retro-transcribed and amplified by PCR the full-length cDNAs specifying the rat and human precursors of the single-stranded mitochondrial DNA (mtDNA)-binding protein (mtSSB). Each deduced sequence is composed of a 16-amino-acid (aa) N-terminal basic pre-sequence and a mature protein (132 aa in humans and 135 aa in the rat). The mature proteins are highly conserved among themselves and with the mtSSB from Xenopus laevis (Xl). Moreover, three regions of the protein are similar to corresponding domains of the SSB of Escherichia coli and to the E. coli F-sex factor SSB, indicating the existence of a broad class of DNA-binding proteins with structural and functional similarities both in prokaryotes and in prokaryote-derived organelles of higher organisms.

引用

页码：219 / 225

页数：7

共 42 条

[1] MODULATION OF THE AFFINITY OF THE SINGLE-STRANDED DNA-BINDING PROTEIN OF ESCHERICHIA-COLI (ESCHERICHIA-COLI SSB) TO POLY(DT) BY SITE-DIRECTED MUTAGENESIS [J].