IN-VITRO FERTILIZATION OF ANDROGEN STERILIZED MICE

被引：11

作者：

KAMIJO, T ^{[1
]}

MIZUNUMA, H ^{[1
]}

YAMADA, K ^{[1
]}

IBUKI, Y ^{[1
]}

机构：

[1] GUNMA UNIV,SCH MED,DEPT OBSTET & GYNECOL,MAEBASHI,GUMMA 371,JAPAN

来源：

LIFE SCIENCES | 1994年 / 55卷 / 07期

关键词：

IN VITRO FERTILIZATION; ANDROGEN-STERILIZED MICE; PREGNANT MARE SERUM GONADOTROPIN;

D O I：

10.1016/0024-3205(94)00745-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

In order to explore the effect of neonatal androgen administration on oocyte quality, ovulation induction and in vitro fertilization (IVF) were performed in androgen-sterilized mice. Androgen sterilized mice were produced by the subcutaneous injection of testosterone propionate (TP) at 5 days of age and ovulation induction was performed by pregnant mare's serum gonadotropin (PMSG)-human chorionic gonadotropin (hCG) treatment at 9 to 13weeks old. The number of oocytes ovulated in TP-injected mice was 19.9 +/- 2.0 (Mean +/- SE) and was significantly less than that in normal mice (37.8 +/- 1.9; P<0.01). The fertilization rate of oocytes retrieved from TP-treated mice (38.8%) was significantly lower than that from normal mice (60.3%; P<0.01). These results indicate that neonatal androgen treatment has a detrimental effect on oocyte maturity.

引用

页码：527 / 531

页数：5

共 15 条

[1] Barraclough C A, 1966, Recent Prog Horm Res, V22, P503
[2] BARRACLOUGH CA, 1954, P SOC EXP BIOL MED, V85, P673, DOI 10.3181/00379727-85-20989
[3] BARRACLOUGH CA, 1968, P SOC EXP BIOL MED, V128, P781
[4] BORENDEG J, 1972, J ENDOCRINOL, V55, P207
[5] GATES A. H., 1971, Methods in mammalian embryology, P64
[6] HAHN DW, 1978, ENDOCRINOLOGY, V102, P1741
[7] HEIDI S, 1988, BIOL REPROD, V38, P636
[8] HOGAN B, 1986, MANIPULATING MOUSE E, P90
[9] KRAMEN MA, 1971, FERTIL STERIL, V22, P745
[10] POLYCYSTIC OVARIAN DISEASE - ANIMAL-MODELS
MAHAJAN, DK
[J]. ENDOCRINOLOGY AND METABOLISM CLINICS OF NORTH AMERICA, 1988, 17 (04) : 705 - 732

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