DETERMINATION OF CARBOFURAN IN WATER AND SOIL BY A RAPID MAGNETIC PARTICLE-BASED ELISA

被引：21

作者：

JOURDAN, SW ^{[1
]}

SCUTELLARO, AM ^{[1
]}

FLEEKER, JR ^{[1
]}

HERZOG, DP ^{[1
]}

RUBIO, FM ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT BIOCHEM,FARGO,ND 58105

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 10期

关键词：

CARBOFURAN; CARBAMATE; IMMUNOASSAY; ELISA; WATER; SOIL;

D O I：

10.1021/jf00058a044

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of carbofuran in water and soil was developed using a magnetic particle-based solid phase. This assay utilizes magnetic particles as the solid phase to attach polyclonal rabbit anti-carbofuran antibodies. The ELISA has an estimated detection limit of 0.056 parts per billion (ppb, ng/mL) of carbofuran in water and 5.6 ppb in soil. The standard curve allows quantitation up to 5.0 ppb in water and 500 ppb in soil. The ELISA compares favorably with HPLC measurements in the analysis of water samples (r = 0.967). Recoveries from soils fortified with 0.05-1 ppm of carbofuran averaged 98.6% using l-h extractions with methanol/water (75:25 v/v).

引用

页码：2784 / 2788

页数：5

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