BIOCHEMICAL-PROPERTIES AND LOCALIZATION OF THE CHROMOSOMAL PROTEIN-IP25

被引:32
作者
KEPPEL, F [1 ]
ALLET, B [1 ]
EISEN, H [1 ]
机构
[1] UNIV GENEVA,DEPT MOLEC BIOL,CH-1211 GENEVA 4,SWITZERLAND
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 96卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1979.tb13060.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The protein IP25, which has previously been reported to accumulate in the chromatin during erythroid differentiation of Friend‐virus‐transformed erythroleukemia cells (FL cells), is shown to behave like histone H1 without being structurally related to it. Like H1. IP25 is not released by digestion of FL cells nuclei with DNAse I. After micrococcal digestion IP25 and HI are differentially distributed in the nucleosome monomers and dimers. This distribution suggests an internucleosomal location for IP25 and H1. Different rates of digestion are observed between nuclei of differentiating and non‐differentiating FL cells with both DNAse I and micrococcal nuclease. These differences could be due to the presence of IP25 in the chromatin of differentiating cells. Copyright © 1979, Wiley Blackwell. All rights reserved
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页码:477 / 482
页数:6
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