CHARACTERIZATION OF VIP-PREFERRING AND HELODERMIN-PREFERRING RECEPTORS ON HUMAN SMALL-CELL LUNG-CARCINOMA CELL-LINES

被引：41

作者：

LUIS, J

SAID, SI

机构：

[1] UNIV ILLINOIS, CHICAGO, IL 60612 USA

[2] VET ADM W SIDE MED CTR, CHICAGO, IL 60680 USA

来源：

PEPTIDES | 1990年 / 11卷 / 06期

关键词：

VIP; VIP RECEPTOR; HELODERMIN-LIKE PEPTIDES; HELODERMIN RECEPTOR; SMALL CELL LUNG CARCINOMA; SCLC CELL LINES; MOLECULAR CHARACTERIZATION; CROSS-LINKING;

D O I：

10.1016/0196-9781(90)90158-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the molecular and pharmacologic characteristics of VIP receptors on two human SCLC cell lines: NCI-N592 and NCI-H345. With NCI-N592 cell, the order of potency of VIP-related peptides in inhibiting I-125-VIP binding and in stimulating cAMP production was typical of the human VIP receptor. By covalent cross-linking, a polypeptide of Mr 62,300 was obtained. Conversely, the behavior of NCI-H345 cell line was totally different: helodermin was the most potent peptide, VIP and PHI were equipotent, while hGRF and secretin were totally ineffective. These results suggest that NCI-N592 cells possess a typical VIP receptor while NCI-H345 cells possess a helodermin-preferring receptor, and that the natural target of helodermin might not be the VIP receptor.

引用

页码：1239 / 1244

页数：6

共 35 条

[1] ABE K, 1984, ENDOCRINE LUNG HLTH, P549
[2] VASOACTIVE INTESTINAL POLYPEPTIDE INCREASES INOSITOL PHOSPHOLIPID BREAKDOWN IN THE RAT SUPERIOR CERVICAL-GANGLION
AUDIGIER, S
BARBERIS, C
JARD, S
[J]. BRAIN RESEARCH, 1986, 376 (02) : 363 - 367
[3] INTERACTIONS OF GLUCAGON, GUT GLUCAGON, VASOACTIVE INTESTINAL POLYPEPTIDE AND SECRETIN WITH LIVER AND FAT-CELL PLASMA-MEMBRANES - BINDING TO SPECIFIC SITES AND STIMULATION OF ADENYLATE CYCLASE
BATAILLE, D
FREYCHET, P
ROSSELIN, G
[J]. ENDOCRINOLOGY, 1974, 95 (03) : 713 - 721
[4] CARNEY DN, 1985, CANCER RES, V45, P2913
[5] BOMBESIN-LIKE PEPTIDES CAN FUNCTION AS AUTOCRINE GROWTH-FACTORS IN HUMAN SMALL-CELL LUNG-CANCER
CUTTITTA, F
CARNEY, DN
MULSHINE, J
MOODY, TW
FEDORKO, J
FISCHLER, A
MINNA, JD
[J]. NATURE, 1985, 316 (6031) : 823 - 826
[6] VASOACTIVE INTESTINAL POLYPEPTIDE AND GLUCAGON - STIMULATION OF ADENYLATE CYCLASE ACTIVITY VIA DISTINCT RECEPTORS IN LIVER AND FAT-CELL MEMBRANES
DESBUQUOIS, B
LAUDAT, MH
LAUDAT, P
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 53 (04) : 1187 - 1194
[7] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP) RECEPTORS IN MAMMALIAN LUNG
DICKINSON, KEJ
SCHACHTER, M
MILES, CMM
COY, DH
SEVER, PS
[J]. PEPTIDES, 1986, 7 (05) : 791 - 800
[8] THE VASOACTIVE-INTESTINAL-PEPTIDE RECEPTOR ON INTACT HUMAN COLONIC ADENOCARCINOMA CELLS (HT29-D4) - EVIDENCE FOR ITS GLYCOPROTEIN NATURE
ELBATTARI, A
LUIS, J
MARTIN, JM
FANTINI, J
MULLER, JM
MARVALDI, J
PICHON, J
[J]. BIOCHEMICAL JOURNAL, 1987, 242 (01) : 185 - 191
[9] ELBATTARI A, 1988, ANN NY ACAD SCI, V527, P667
[10] FRAKER PJ, 1978, BIOCHEM BIOPH RES CO, V80, P849, DOI 10.1016/0006-291X(78)91322-0

← 1 2 3 4 →