EXTRACTION AND QUANTITATION OF NEUROPEPTIDES IN BONE BY RADIOIMMUNOASSAY

被引：47

作者：

AHMED, M ^{[1
]}

SRINIVASAN, GR ^{[1
]}

THEODORSSON, E ^{[1
]}

BJURHOLM, A ^{[1
]}

KREICBERGS, A ^{[1
]}

机构：

[1] KAROLINSKA HOSP, DEPT CLIN CHEM, S-10401 STOCKHOLM, SWEDEN

来源：

REGULATORY PEPTIDES | 1994年 / 51卷 / 03期

关键词：

SUBSTANCE P; CALCITONIN GENE-RELATED PEPTIDE; NEUROPEPTIDE Y; VASOACTIVE INTESTINAL POLYPEPTIDE; BONE TISSUE; RAT;

D O I：

10.1016/0167-0115(94)90063-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The feasibility of extracting and quantifying neuropeptides in bone by radioimmunoassay was investigated in a study including 60 diaphyseal rat femora. Substance P, calcitonin gene-related peptide, neuropeptide Y and vasoactive intestinal polypeptide, previously identified in bone by immunohistochemistry, were extracted from separate homogenates of bone, periosteum and bone marrow in a solution of 4% EDTA and 2 M acetic acid. Measurable amounts of all four neuropeptides in bone, periosteum and bone marrow were obtained by radioimmunoassay in a reproducible manner. The neuropeptide immunoreactivities were characterized by reverse-phase high performance liquid chromatography. Among the four neuropeptides analyzed, neuropeptide Y consistently exhibited the highest concentrations in the different tissues. Overall, cortical bone showed the lowest neuropeptide concentrations. The concentration of vasoactive intestinal polypeptide was higher in periosteum than in bone marrow, whereas that of calcitonin gene-related peptide was uniform in these tissues. The distributional differences observed in bone tissue may be explained by a variety of physiological roles attributed to neuropeptides such as regulation of nociception, vasoactivity, immune function and local bone metabolism. The described methodology offers a new means of investigating a neuropeptidergic involvement in various disorders of the skeleton.

引用

页码：179 / 188

页数：10

共 45 条

[1]

AHMED M, 1993, IN PRESS PEPTIDE

[2]

BJURHOLM A, 1992, J BONE MINER RES, V7, P1011

[3] FIXATION AND DEMINERALIZATION OF BONE TISSUE FOR IMMUNOHISTOCHEMICAL STAINING OF NEUROPEPTIDES [J].

BJURHOLM, A ;

KREICBERGS, A ;

SCHULTZBERG, M .

CALCIFIED TISSUE INTERNATIONAL, 1989, 45 (04) :227-231

[4] PARATHYROID-HORMONE AND NORADRENALINE-INDUCED ENHANCEMENT OF CYCLIC-AMP IN A CLONED OSTEOGENIC-SARCOMA CELL-LINE (UMR-106) IS INHIBITED BY NEUROPEPTIDE-Y [J].

BJURHOLM, A ;

KREICBERGS, A ;

SCHULTZBERG, M ;

LERNER, UH .

ACTA PHYSIOLOGICA SCANDINAVICA, 1988, 134 (03) :451-452

[5] SUBSTANCE-P-IMMUNOREACTIVE AND CGRP-IMMUNOREACTIVE NERVES IN BONE [J].

BJURHOLM, A ;

KREICBERGS, A ;

BRODIN, E ;

SCHULTZBERG, M .

PEPTIDES, 1988, 9 (01) :165-171

[6] NEUROPEPTIDE Y-IMMUNOREACTIVE, TYROSINE HYDROXYLASE-IMMUNOREACTIVE AND VASOACTIVE INTESTINAL POLYPEPTIDE-IMMUNOREACTIVE NERVES IN BONE AND SURROUNDING TISSUES [J].

BJURHOLM, A ;

KREICBERGS, A ;

TERENIUS, L ;

GOLDSTEIN, M ;

SCHULTZBERG, M .

JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1988, 25 (2-3) :119-125

[7] CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR [J].

BRAIN, SD ;

WILLIAMS, TJ ;

TIPPINS, JR ;

MORRIS, HR ;

MACINTYRE, I .

NATURE, 1985, 313 (5997) :54-56

[8] TACHYKININ MULTIPLICITY IN RAT CENTRAL-NERVOUS-SYSTEM AS STUDIED USING ANTISERA RAISED AGAINST SUBSTANCE-P AND NEUROKININ-A [J].

BRODIN, E ;

LINDEFORS, N ;

DALSGAARD, CJ ;

THEODORSSONNORHEIM, E ;

ROSELL, S .

REGULATORY PEPTIDES, 1986, 13 (3-4) :253-272

[9]

CASINI A, 1989, N-S ARCH PHARMACOL, V339, P354

[10] HUMAN SYNTHETIC CALCITONIN GENE-RELATED PEPTIDE INHIBITS BONE-RESORPTION INVITRO [J].

DSOUZA, SM ;

MACINTYRE, I ;

GIRGIS, SI ;

MUNDY, GR .

ENDOCRINOLOGY, 1986, 119 (01) :58-61

← 1 2 3 4 5 →