THERMODYNAMIC BEHAVIOR OF MIXED BENZODIAZEPINES BY A NEW LIQUID-CHROMATOGRAPHIC METHOD

被引：8

作者：

GUILLAUME, Y

GUINCHARD, C

机构：

[1] Faculté des Sciences Médicales et Pharmaceutiques, Laboratoire de Chimie Analytique, Besançon Cedex, 25030, Place St Jacques

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 3-4期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CHEMOMETRIC METHODOLOGY; ELUTION ORDER; SIMPLEX OPTIMIZATION; BENZODIAZEPINES;

D O I：

10.1007/BF02272170

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a rapid chemometric methodology to determine the separation factor, alpha, at different temperatures, Gibbs Helmholtz parameters (Delta(Delta H), Delta(Delta S), Delta(Delta G)) of two adjacent benzodiazepines on a chromatogram were obtained from ln alpha versus T-1 plots. A temperature dependent reversal of the elution order was studied and the mobile phase composition and column temperature were optimized to obtain the best separation. A flow rate of 0.80 ml min(-1) with 52.6% methanol in the methanol-water mixture and a column temperature of 48 degrees C gave the most efficient separation of ten benzodiazepines.

引用

页码：193 / 196

页数：4

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