EVALUATION OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)-PCR AS A METHOD TO DIFFERENTIATE LACTOBACILLUS-ACIDOPHILUS, LACTOBACILLUS-CRISPATUS, LACTOBACILLUS-AMYLOVORUS, LACTOBACILLUS-GALLINARUM, LACTOBACILLUS-GASSERI, AND LACTOBACILLUS-JOHNSONII

被引：86

作者：

DUPLESSIS, EM ^{[1
]}

DICKS, LMT ^{[1
]}

机构：

[1] UNIV STELLENBOSCH,DEPT MICROBIOL,STELLENBOSCH 7600,SOUTH AFRICA

来源：

CURRENT MICROBIOLOGY | 1995年 / 31卷 / 02期

关键词：

D O I：

10.1007/BF00294286

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The technique random amplified polymorphic DNA (RAPD)-PCR was evaluated as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri', and Lactobacillus johnsonii. Representative strains, including the type of each species, were selected from different clusters obtained by numerical analysis of total soluble cell protein patterns. Results obtained by RAPD-PCR corresponded well with results obtained by numerical analysis of total soluble cell protein patterns. The type strains of each species displayed different RAPD profiles. Strains with identical L(+)- nicotinamide adenine dinucleotide-dependent lactic dehydrogenase (nLDH) electrophoretic profiles could be distinguished on the basis of their RAPD profiles.

引用

页码：114 / 118

页数：5

共 13 条

[1] SYNONYMY OF STRAINS OF LACTOBACILLUS-ACIDOPHILUS GROUP-A2 (JOHNSON ET AL 1980) WITH THE TYPE STRAIN OF LACTOBACILLUS-CRISPATUS (BRYGOO AND ALADAME 1953) MOORE AND HOLDEMAN 1970
CATO, EP
MOORE, WEC
JOHNSON, JL
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1983, 33 (02): : 426 - 428
[2] DEOXYRIBONUCLEIC ACID HOMOLOGY AND BASE COMPOSITION IN SOME THERMOPHILIC LACTOBACILLI
DELLAGLIO, F
BOTTAZZI, V
TROVATELLI, LD
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1973, 74 (FEB): : 289 - 297
[3] DESIGNATION OF ATCC 334 IN PLACE OF ATCC 393 (NCDO 161) AS THE NEOTYPE STRAIN OF LACTOBACILLUS-CASEI SUBSP. CASEI AND REJECTION OF THE NAME LACTOBACILLUS-PARACASEI
DELLAGLIO, F
DICKS, LMT
DUTOIT, M
TORRIANI, S
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (02): : 340 - 342
[4] TAXONOMY OF LEUCONOSTOC SPECIES, PARTICULARLY LEUCONOSTOC-OENOS, AS REVEALED BY NUMERICAL-ANALYSIS OF TOTAL SOLUBLE CELL PROTEIN-PATTERNS, DNA-BASE COMPOSITIONS, AND DNA-DNA HYBRIDIZATIONS
DICKS, LMT
VANVUUREN, HJJ
DELLAGLIO, F
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1990, 40 (01): : 83 - 91
[5] DICKS LMT, 1990, FEMS MICROBIOL LETT, V67, P9, DOI 10.1016/S0378-1097(98)00376-0
[6] DICKS LMT, 1993, INT J SYST BACTERIOL, V34, P347
[7] TAXONOMIC STUDY OF THE LACTOBACILLUS-ACIDOPHILUS GROUP, WITH RECOGNITION OF LACTOBACILLUS-GALLINARUM SP-NOV AND LACTOBACILLUS-JOHNSONII SP-NOV AND SYNONYMY OF LACTOBACILLUS-ACIDOPHILUS GROUP-A3 (JOHNSON ET-AL 1980) WITH THE TYPE STRAIN OF LACTOBACILLUS-AMYLOVORUS (NAKAMURA 1981)
FUJISAWA, T
BENNO, Y
YAESHIMA, T
MITSUOKA, T
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03): : 487 - 491
[8] TAXONOMY OF THE LACTOBACILLUS-ACIDOPHILUS GROUP
JOHNSON, JL
PHELPS, CF
CUMMINS, CS
LONDON, J
GASSER, F
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1980, 30 (01): : 53 - 68
[9] KANDLER O, 1986, BERGEYS MANUAL SYSTE, V2, P1209
[10] IDENTIFICATION AND GROUPING OF BACTERIA BY NUMERICAL-ANALYSIS OF THEIR ELECTROPHORETIC PROTEIN PATTERNS
KERSTERS, K
DELEY, J
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1975, 87 (APR): : 333 - 342

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