DIRECT CONVERSION OF DOPAMINE 3-0-SULFATE TO NOREPINEPHRINE BY DOPAMINE-BETA-HYDROXYLASE

被引：67

作者：

BUU, NT

KUCHEL, O

机构：

[1] Clinical Research Institute of Montreal, Montreal, Que. H2W 1R7

来源：

LIFE SCIENCES | 1979年 / 24卷 / 09期

关键词：

D O I：

10.1016/0024-3205(79)90361-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Dopamine 3-0-sulfate is present in considerable amounts in mammalian plasma and peripheral tissues. Incubation of dopamine 3-0-sulfate (0.1 μmole) with purified bovine dopamine-β-hydroxylase resulted in the formation of free norepinephrine (7.3 × 10-3 μmole). The conversion to norepinephrine was inhibited by 0.6 mM of fusaric acid, an inhibitor of dopamine-β-hydroxylase. The reaction of dopamine 3-0-sulfate with dopamine-β-hydroxylase followed Michaelis-Menten kinetics. The calculated Km was 17 mM, different from the Km for free dopamine (0.1 mM). The incubation medium does not contain any sulfatase activity. © 1979.

引用

页码：783 / 789

页数：7

共 23 条

[1] BOVINE ADRENAL MEDULLARY DOPAMINE-BETA-HYDROXYLASE - STUDIES ON INTERACTION WITH CONCANAVALIN A [J].