EFFICIENCY OF PARTICLE-BOMBARDMENT-MEDIATED TRANSFORMATION IS INFLUENCED BY CELL-CYCLE STAGE IN SYNCHRONIZED CULTURED-CELLS OF TOBACCO

被引：37

作者：

IIDA, A

YAMASHITA, T

YAMADA, Y

MORIKAWA, H ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC SCI,DEPT BIOL SCI,HIGASHI KU,HIROSHIMA 724,JAPAN

[2] SUMITOMO CHEM CO LTD,BIOTECHNOL LAB,TAKARAZUKA RES CTR,TAKARAZUKA,HYOGO 665,JAPAN

[3] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,KYOTO 606,JAPAN

来源：

PLANT PHYSIOLOGY | 1991年 / 97卷 / 04期

关键词：

D O I：

10.1104/pp.97.4.1585

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plasmid DNA pBI221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G2 phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G1 phases.

引用

页码：1585 / 1587

页数：3

共 7 条

[1]

IIDA A, 1990, APPL MICROBIOL BIOT, V33, P560, DOI 10.1007/BF00172551

[2] GENE DELIVERY INTO CULTURED PLANT-CELLS BY DNA-COATED GOLD PARTICLES ACCELERATED BY A PNEUMATIC PARTICLE GUN [J].