CONSTRUCTION AND PHYSIOLOGICAL CHARACTERIZATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OVERPRODUCING TRANSFORMANTS OF ASPERGILLUS-NIDULANS

被引：16

作者：

HANEGRAAF, PPF ^{[1
]}

PUNT, PJ ^{[1
]}

VANDENHONDEL, CAMJJ ^{[1
]}

DEKKER, J ^{[1
]}

YAP, W ^{[1
]}

VANVERSEVELD, HW ^{[1
]}

STOUTHAMER, AH ^{[1
]}

机构：

[1] TNO,RR54F,2280 HV RIJSWIJK,NETHERLANDS

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 34卷 / 06期

关键词：

D O I：

10.1007/BF00169347

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The construction and characterization of glyceraldehyde-3-phosphate-dehydrogenase (GPD) overproducing transformants of Aspergillus nidulans and their behaviour in acetate-limited continuous cultures and glucose-grown batch cultures are described. The A. nidulans acetamidase deletion strain MH1277 was transformed with the homologous gpdA gene on a vector with the homologous acetamidase-gene (amdS) as a selection marker. Transformant Al contains about nine integrated copies of the gpdA gene, and shows a proportional gene-dosage GPD production of about 22% of the total soluble cell protein. Compared to the wild-type MH1277, Al has higher growth yields and reaches higher specific growth rates on both acetate and glucose, which could be due to the key position of GDP in glycolysis and gluconeogenesis.

引用

页码：765 / 771

页数：7

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