ADSORPTION AND EXPRESSION OF PENICILLIN-G ACYLASE IMMOBILIZED ONTO METHACRYLATE POLYMERS GENERATED WITH VARYING PORE GENERATING SOLVENT VOLUME

被引：12

作者：

KOTHA, A

SELVARAJ, L

RAJAN, CR

PONRATHNAM, S

KUMAR, KK

AMBEKAR, GR

SHEWALE, JG

机构：

[1] HINDUSTAN ANTIBIOT LTD,RES & DEV,POONA 411018,INDIA

[2] NATL CHEM LAB,DIV CHEM ENGN,POONA 411008,MAHARASHTRA,INDIA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 30卷 / 03期

关键词：

PENICILLIN-G ACYLASE; IMMOBILIZATION; METHACRYLATE POLYMER BEADS;

D O I：

10.1007/BF02922033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adsorption and expression of penicillin G acylase was studied on macroporous methacrylate polymer beads of differing pore volume, generated with kerosene. The absorption and expression of the penicillin G acylase was dependent on pore volume. Maximum expression of 57% of adsorbed enzyme was obtained on beads synthesized with 40 mL of kerosene, indicating minimum pore-diffusion limitations.

引用

页码：297 / 302

页数：6

共 10 条

[1]

Borkar P S, 1978, Hindustan Antibiot Bull, V20, P81

[2] KINETIC-BEHAVIOR OF IMMOBILIZED PENICILLIN ACYLASE [J].