STRUCTURAL-ANALYSIS OF THE N-LINKED OLIGOSACCHARIDES FROM MURINE GLYCOPHORIN

被引:11
作者
ANGEL, AS
GRONBERG, G
KROTKIEWSKI, H
LISOWSKA, E
NILSSON, B
机构
[1] BIOCARB TECHNOL AB,S-22370 LUND,SWEDEN
[2] POLISH ACAD SCI,LUDWIK HIRSZFELD INST IMMUNOL & EXPTL THERAPY,DEPT IMMUNOCHEM,PL-53114 WROCLAW,POLAND
关键词
D O I
10.1016/0003-9861(91)90107-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glycophorins, isolated from BALB/c mouse erythrocytes, were degraded under mild and strong reductive alkaline conditions and the N-linked Oligosaccharides were isolated as alditols. The oligosaccharide alditols were fractionated and purified using gel filtration, concanavalin A-Sepharose affinity chromatography, and high-performance ion-exchange chromatography. Structural analysis was carried out by chemical analyses, periodate oxidation in combination with fast atom bombardment mass spectrometry, and 500-MHz 1H NMR spectroscopy. The results revealed the presence of sialylated biantennary, triantennary, and tetraantennary complex type oligosaccharides, all fucosylated at the innermost N-acetylglucosamine residue. The tri- and tetraantennary oligosaccharide-containing fractions also contained species elongated by one and/or two N-acetyllactosamine (-3Galβ1-4GlcNAcβ1-) sequences. The N-linked oligosaccharides were shown to be combined only with one (the low molecular weight) of the two mouse glycophorins. © 1991.
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页码:76 / 88
页数:13
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