2-DIMENSIONAL ELECTROPHORESIS METHOD USED FOR DETERMINATION OF PLASMID PROFILES OF ESCHERICHIA-COLI ISOLATED FROM A SEWAGE-TREATMENT PLANT

被引：3

作者：

ARTURO, M ^{[1
]}

TAMANAISHACOORI, Z ^{[1
]}

MAMEZ, C ^{[1
]}

POMMEPUY, M ^{[1
]}

CORMIER, M ^{[1
]}

机构：

[1] IFREMER, CTR BREST, BACTERIOL LAB, F-29263 PLOUZANE, FRANCE

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1993年 / 39卷 / 10期

关键词：

ESCHERICHIA-COLI; PLASMIDS; 2-DIMENSIONAL ELECTROPHORESIS; SEWAGE TREATMENT; BRACKISH WATER;

D O I：

10.1139/m93-150

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plasmid contents of 306 strains of Escherichia coli isolated from raw and treated sewage from a treatment plant as well as from the water 1 km downstream were determined. The number and molecular weight of plasmids isolated from a representative sample of these strains were also measured. It was observed that biological treatment did not significantly alter these parameters. In contrast, statistically significant differences in plasmid number and size were observed when strains from sewage (raw and treated) were compared with strains isolated from brackish water. In raw and treated sewage, more than 96% of the strains contained plasmids, compared with 85% in brackish water. Nine to 13% of the strains from sewage contained plasmids with more than 56 kilobases, while only 5% of the strains from brackish water reached this size.

引用

页码：990 / 993

页数：4

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