MANNOSE-BINDING PROTEIN RECOGNIZES GLIOMA-CELLS - IN-VITRO ANALYSIS OF COMPLEMENT ACTIVATION ON GLIOMA-CELLS VIA THE LECTIN PATHWAY

被引：38

作者：

FUJITA, T ^{[1
]}

TAIRA, S ^{[1
]}

KODAMA, N ^{[1
]}

MATSUSHITA, M ^{[1
]}

FUJITA, T ^{[1
]}

机构：

[1] FUKUSHIMA MED COLL,DEPT BIOCHEM,FUKUSHIMA 96012,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1995年 / 86卷 / 02期

关键词：

GLIOMA; COMPLEMENT; MANNOSE BINDING PROTEIN; LECTIN PATHWAY; COMPLEMENT REGULATORY FACTOR;

D O I：

10.1111/j.1349-7006.1995.tb03038.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The lectin pathway is a novel pathway far activation of the complement cascade, which is initiated by the binding of mannose-binding protein (MBP) to its carbohydrate ligands. We investigated whether the complement system was activated in vitro by glioma cells through this pathway to the C3 level. MBP was found to bind to all six glioma cell lines tested by using flow cytometric analysis. Binding of a complex of MBP-associated serine protease and MBP was observed in two of the cell lines examined, thereby resulting in C4 consumption. Activation of C3 was hemolytically evaluated in these two lines. C3 consumption was also observed in one. Based on these results, it is likely that recognition by MBP followed by complement activation occurs in certain glioma cell lines.

引用

页码：187 / 192

页数：6

共 25 条

[1] PURIFICATION AND STRUCTURAL-ANALYSIS OF 4TH COMPONENT OF HUMAN COMPLEMENT [J].