Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger

被引：8

作者：

Ramadas, M ^{[1
]}

Holst, O ^{[1
]}

Mattiasson, B ^{[1
]}

机构：

[1] LUND UNIV,CTR CHEM,DEPT BIOTECHNOL,S-22100 LUND,SWEDEN

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 12期

关键词：

D O I：

10.1007/BF00158544

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aqueous two-phase extraction was employed, as a new approach,for the recovery of amyloglucosidase (AMG) after solid state fermentation. In an aqueous two-phase system of PEG 6000 and potassium phosphate, 95% of the AMG was recovered in the bottom salt phase, with all visible particles from solid fungal bran in the interface, giving a purification factor of 11. Affinity purification of AMG was also done by adsorption onto crosslinked starch, in the presence of PEG or salt. Both were found to: enhance the adsorption. In the presence of PEG 6000 (10% w/w), two fold increase in AMG adsorption, 85% of AMG recovery and 6.0 fold of purification were achieved. The purified AMG was found homogeneous by SDS polyacrylamide gel electrophoresis.

引用

页码：901 / 906

页数：6

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