TESTOSTERONE REGULATES PROGONADOTROPIN-RELEASING HORMONE LEVELS IN THE PREOPTIC AREA AND BASAL HYPOTHALAMUS OF THE MALE-RAT

被引:48
作者
ROSELLI, CE
KELLY, MJ
RONNEKLEIV, OK
机构
[1] Department of Physiology, Oregon Health Sciences University
[2] Department of Physiology, Oregon Health Sciences University, Portland, OR
关键词
D O I
10.1210/endo-126-2-1080
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
GnRH is synthesized as part of a larger 92- amino acid prohormone (pro-GnRH). In the present study, we examined the effect of gonadectomy and hormone replacement on the processing of pro-GnRH to GnRH in adult male rats. Immunoreactive levels of pro-GnRH and GnRH in acid extracts of preoptic area (POA) and basal hypothalamus (BH) from intact, castrate, and testosterone (T)-treated castrate males were quantified by RIA. In addition, we used immunocytochemistry to detect pro-GnRH-and GnRH-positive neurons and determine the effects of hormone treatment. Three weeks after castration, the pro-GnRH content of the POA was 2-fold greater than the amount found in intact males. Treatment with T for 1 week lowered the prohormone content to intact levels. Although the pro-GnRH content in the BH was about 50% lower than that in the POA, the BH responded to castration and steroid replacement in a manner identical to the POA. The GnRH content of the BH, but not that of the POA, was significantly reduced after castration and increased by T treatment. On the other hand, the total number of pro-GnRH and GnRH cell bodies visualized by immunocytochemistry was not significantly altered by either treatment. These results show that changes in pro-GnRH content vary in inverse relation to changes in GnRH content and suggest that gonadectomy inhibits the enzymatic processing of precursor, while T treatment promotes it. © 1990 by The Endocrine Society.
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页码:1080 / 1086
页数:7
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