HEPATITIS-C VIRUS IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS

被引：30

作者：

HSIEH, TT

YAO, DS

SHEEN, IS

LIAW, YF

PAO, CC

机构：

[1] CHANG GUNG MED COLL,DEPT BIOCHEM,259 WENHWA RD,TAOYWAN,TAIWAN

[2] CHANG GUNG MEM HOSP,DEPT OBSTET & GYNECOL,TAIPEI,TAIWAN

[3] CHANG GUNG MEM HOSP,LIVER UNIT,TAIPEI,TAIWAN

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1992年 / 98卷 / 04期

关键词：

HEPATITIS-C VIRUS; REVERSE TRANSCRIPTION; POLYMERASE CHAIN REACTION; PERIPHERAL BLOOD MONONUCLEAR CELLS;

D O I：

10.1093/ajcp/98.4.392

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

RNA extracted from plasma and peripheral blood mononuclear cells of patients with chronic hepatitis C were used as the template for reverse transcription followed by double in vitro enzymatic amplification with nested primers. Hepatitis C virus was detected in 14 of 15 (93.3%) plasma specimens and in 8 of 15 (53.3%) peripheral blood mononuclear cell specimens obtained from patients with chronic hepatitis C and abnormal liver functions. The results suggest that hepatitis C virus could be found frequently in peripheral blood mononuclear cells of patients with chronic hepatitis C. Whether the presence of hepatitis C virus in peripheral blood mononuclear cells plays any role in the pathogenesis of diseases associated with hepatitis C virus infection remains to be determined.

引用

收藏

页码：392 / 396

页数：5

相关论文

共 16 条

[1] HEPATITIS-C VIRUS-INFECTION IN POSTTRANSFUSION HEPATITIS - AN ANALYSIS WITH 1ST-GENERATION AND 2ND-GENERATION ASSAYS [J].

AACH, RD ;

STEVENS, CE ;

HOLLINGER, FB ;

MOSLEY, JW ;

PETERSON, DA ;

TAYLOR, PE ;

JOHNSON, RG ;

BARBOSA, LH ;

NEMO, GJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (19) :1325-1329

[2] DETECTION OF ANTIBODY TO HEPATITIS-C VIRUS IN PROSPECTIVELY FOLLOWED TRANSFUSION RECIPIENTS WITH ACUTE AND CHRONIC NON-A-HEPATITIS, NON-B-HEPATITIS [J].

ALTER, HJ ;

PURCELL, RH ;

SHIH, JW ;

MELPOLDER, JC ;

HOUGHTON, M ;

CHOO, QL ;

KUO, G .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (22) :1494-1500

[3] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[4] HEPATITIS-C VIRAL-RNA IN SERUM OF PATIENTS WITH CHRONIC NON-A, NON-B HEPATITIS - DETECTION BY THE POLYMERASE CHAIN-REACTION USING MULTIPLE PRIMER SETS [J].

CRISTIANO, K ;

DIBISCEGLIE, AM ;

HOOFNAGLE, JH ;

FEINSTONE, SM .

HEPATOLOGY, 1991, 14 (01) :51-55

[5] DETECTION OF HEPATITIS-C VIRAL SEQUENCES IN BLOOD DONATIONS BY NESTED POLYMERASE CHAIN-REACTION AND PREDICTION OF INFECTIVITY [J].

GARSON, JA ;

TEDDER, RS ;

BRIGGS, M ;

TUKE, P ;

GLAZEBROOK, JA ;

TRUTE, A ;

PARKER, D ;

BARBARA, JAJ ;

CONTRERAS, M ;

ALOYSIUS, S .

LANCET, 1990, 335 (8703) :1419-1422

[6] DEMONSTRATION OF VIREMIA PATTERNS IN HEMOPHILIACS TREATED WITH HEPATITIS-C-VIRUS-CONTAMINATED FACTOR-VIII CONCENTRATES [J].

GARSON, JA ;

TUKE, PW ;

MAKRIS, M ;

BRIGGS, M ;

MACHIN, SJ ;

PRESTON, FE ;

TEDDER, RS .

LANCET, 1990, 336 (8722) :1022-1025

[7]

Higuchi R, 1989, PCR TECHNOLOGY, P31

[8] IMPROVED SERODIAGNOSIS OF HEPATITIS-C VIRUS-INFECTION WITH SYNTHETIC PEPTIDE ANTIGEN FROM CAPSID PROTEIN [J].

HOSEIN, B ;

FANG, CT ;

POPOVSKY, MA ;

YE, J ;

ZHANG, ML ;

WANG, CY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3647-3651

[9] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].

KUO, G ;

CHOO, QL ;

ALTER, HJ ;

GITNICK, GL ;

REDEKER, AG ;

PURCELL, RH ;

MIYAMURA, T ;

DIENSTAG, JL ;

ALTER, MJ ;

STEVENS, CE ;

TEGTMEIER, GE ;

BONINO, F ;

COLOMBO, M ;

LEE, WS ;

KUO, C ;

BERGER, K ;

SHUSTER, JR ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :362-364

[10]

OKAMOTO H, 1990, JPN J EXP MED, V60, P215